Cytoplasm Biophysical Properties Limit Cytoskeleton Dynamics In Vivo

The cytoplasm is a very crowded environment harboring most of the metabolic reactions necessary for cellular function. Its biophysical properties are influenced by metabolism. Indeed, changes in mechanical have been linked to differentiation and concentration cell death. However, reverse relationship has not extensively studied. Here we tried address effects on cytoskeleton dynamics. Our results demonstrate sensitivity dynamics, specifically concentration. In fission yeast, changing cytoplasmic density reversibly affects dynamic processes such as actin microtubule We found that inversely correlated density. Increasing slowed down growth shrinkage rates dose-dependent manner. This effect dynamics did depend stress pathway. extended our observation plants confirmed suggesting probing an inherent property cytoplasm. discovered can be alleviated diluting he increase set out explore vitro growing buffers various viscosity obtained supporting evidence. highlight how important metabolism eukaryotes suggest limits fast happen cells. study reveals interplay between biology physics happening assay first step understanding affect biological it encloses.